![]() ![]() One sequence for each group was submitted to the European Molecular Biology Laboratory database (HG793393–HG793394). gondii DNA from all analyzed lungs, resulting in a group of 16 identical sequences and a group of three identical sequences. When the nutria fur market collapsed in the 1940s, thousands of nutria escaped or were released into the wild by ranchers who could no longer afford to feed and house them. The PCR products were purified (Wizard® SV gel, Promega, Madison, Wisconsin, USA) and sent to a commercial sequencing service (Primm Biotech, Milan, Italy). The nutria ( Myocastor coypus ), a large, semi-aquatic rodent native to South America, was originally brought to the United States in 1889 for its fur. Additionally, nutria carry tapeworms, a nematode that causes a rash known as nutria itch, and blood and liver flukes, which can contaminate swimming areas and drinking water supplies. gondii infection (as determined by ELISA) using QIAampDNA Mini Kit (QIAGEN, Valencia, California, USA) following the manufacturer’s instructions and processed DNA by real-time PCR as described by Hurtado et al. ![]() We extracted DNA from lung tissues of 19 coypus with acute T. Antibody titers corresponding to an acute (sample index >200) or chronic infection (sample index 50–200) were observed in 19 and 18 coypus, respectively. (New band name, anyone) Yet, nutria's orange teeth and prolific nature have made. Thirty-seven of 128 (28.9%) animals had detectable antibodies to T. Nutria are terrible for our coastline and can carry a nematode known to cause a rash called nutria itch. An indirect enzyme-linked immunosorbent assay (ELISA) (ID Screen® Toxoplasmosis Indirect Kit, IDVET, Montpellier, France) was used to detect serum antibodies to Toxoplasma gondii. ![]()
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